Cloning of a Full Length cDNA of Human Thrombopoietin Receptor c-Mpl and Construction of Engineered Cells that Stably Express c-mpl
- VernacularTitle:人促血小板生成素受体c-Mpl编码区全长cDNA的克隆及其稳定表达的细胞株的构建
- Author:
Xin-Yan ZHAO
1
;
Jing-Li CAI
;
Wei-Lie DAI
;
Wei-Guo ZHOU
;
Chang-Ben LI
;
Shou-Yuan ZHAO
Author Information
1. 复旦大学
- Keywords:
Human thrombopoietin receptor c-Mpl;
RT－PCR;
transfection;
K562 cell;
expression
- From:
Chinese Journal of Biotechnology
2000;16(3):320-323
- CountryChina
- Language:Chinese
-
Abstract:
A full length cDNA fragment encoding for human thrombopoietin receptor c-Mpl has been amplified by RT PCR from the total RNA of human HEL cells. The complete sequence of the cloned cDNA was determined and is identical to that previously reported. Then the fragment was subcloned into the mammalian expression vector pcDNA3 and the resulting plasmid is designated as pcMPL. K562 cells, which do not express c-mpl, were transfected with pcMPL and pcDNA3, respectively. The transformants were selected with G418 and then tested by Northern and Southern blotting. A group of engineered cell lines stably expressing c-mpl have been obtained,which will facilitate further research on the signaling mediated by c-Mpl.
