Correlation Analysis of Endophytes and Key Secondary Metabolites of Artemisia indica
- VernacularTitle:五月艾内生菌与其关键次生代谢产物的相关性分析
- Author:
Mengzhi LI
1
,
2
;
Peng LIANG
;
Lipeng KANG
;
Chao LI
;
Kun BAI
;
Yanqiu MAO
;
Xianzhang HUANG
Author Information
- Keywords: Artemisia indica; Developmental stages; Endophytes; Diversity; Key secondary metabolites
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(8):2175-2186
- CountryChina
- Language:Chinese
- Abstract: Objective To characterize the temporal dynamics of endophytic community and key metabolites during the growth developmental stages of Artemisia indica and to explore the effects of endophytes on the medicinal material quality of A.indica from the perspective of microorganism combined with the correlation analysis.Methods In this study,high-throughput sequencing was performed to obtain the temporal dynamics of diversity and composition of endophytic community during the growth stages of A.indica.Key metabolites quantitative analysis were performed using high-performance liquid chromatography and ultraviolet spectrophotometry.Spearman's correlation analysis was further conducted to identify the endophytic communities that were significantly correlated with metabolites.Results There were significant differences in the diversity and composition of endophytic communities during the growth stages of A.indica.Linear discriminant analysis efect size(LEfSe)analyses identified some potential microbial biomarkers,such as Firmicutes,Massilia,Pantoea,Alternaria,Didymella,and Trichomerium.The abundances of some genera were significantly and positively correlated with the content of total polyphenols and total flavonoids(P<0.05),such as Curtobacterium,Pantoea,Tilletiopsis,and Dissoconium.The abundances of Pseudozyma,Ralstonia,and Pleospora were significantly and positively correlated with Chlorogenic acid,Isochlorogenic acid B,Isochlorogenic acid A,and Isochlorogenic acid C(P<0.05).Conclusion This work described the distribution of endophytes and key metabolites during the development stages of A.indica.Some endophytic communities with significant correlation of key metabolites were identified,which provided valuable information to guide the isolation of endophytic strains related to key metabolites and improve the quality of A.indica.
