Rapid Analysis of Components in Longshengzhi Capsules Based on UPLC-Q/TOF-MS Technology
- VernacularTitle:基于UPLC-Q/TOF-MS技术的龙生蛭胶囊成分快速分析
- Author:
Mengyi ZHAO
1
;
Chenghao BI
;
Mengjiao LI
;
Zhengwei DONG
;
Jiawei FAN
;
Jinyue ZHANG
Author Information
- Keywords: Longshengzhi capsules; Traditional Chinese medicine prescription; Chemical composition; High performance liquid chromatography; Quadrupole time-of-flight mass spectrometry
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(8):2144-2153
- CountryChina
- Language:Chinese
- Abstract: Objective To establish a rapid detection and identification method for the chemical components of Longshengzhi capsules based on UPLC-Q-TOF/MS.Method UPLC-Q/TOF-MS technology was used to analyze and identify the chemical components of Longshengzhi capsules.The chromatographic column was a Waters Acquity UPLC BEH C18 column(100mm×2.1mm,1.7 μm),and the mobile phase A was 0.1%formic acid water.The mobile phase B was a methanol acetonitrile(1∶1)solution containing 0.1%formic acid(B).The flow rate is 0.3mL/min,the column temperature is 40℃,and the injection volume is 2 μL.The ion source adopts the electric spray ion source.The data is collected in the positive and negative ion modes,and the collection range is m/z 50～1200.The identification and matching are carried out through UNIFI software combined with manual verification of chemical components.Result 87 chemical components were preliminarily and rapidly identified.Conclusion The established method can systematically and rapidly analyze the chemical components in Longsheng leeches,providing a basis for the study of medicinal substances and having important significance for the quality control of Longsheng leeches.
