Curcumol Alleviate Erastin-Induced Ferroptosis in HT22 Cells
- VernacularTitle:姜黄醇通过抑制铁死亡途径减轻HT22小鼠海马神经元细胞损伤
- Author:
Yue LI
1
;
Liang LIANG
;
Junyu CHEN
Author Information
- Keywords: Ferroptosis; Hippocampal neurons of HT22 mice; Curcumol; Oxidative stress injury
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(8):2115-2124
- CountryChina
- Language:Chinese
- Abstract: Objective To verify that curcumol can inhibit erastin-induced ferroptosis in HT22 cells and play a role of cell protection.Methods Firstly,the effect of 0.5 μmol·L-1 Erastin at different time gradients(0,4,8,12,24 h)on the expression of ferroptosis-related proteins in HT22 cells were abserved by Western blot,Then HT22 cells were divided into normal control group,DMSO group,Erastin treatment group(0.5 μmol·L-1 Erastin treatment for 24),and curcumol group(0.5 μmol·L-1 Erastin treatment for 24 h+50 μmol·L-1 curcumin treatment for 48 ho).The expression levels of ferroptosis-related proteins in HT22 cells were detected by Western blot,cell iron ion kit and cell ferrous content kit were used to detect the levels of total iron and ferrous iron in cells,cell viability was detected by CCK-8 kit,and cell apoptosis was detected by Annexin V/PI apoptosis kit.Results After Erastin stimulation,the levels of xCT(P<0.05),NCOA4(P<0.001)and ACSL4(P<0.01)were up-regulated,while GPX4(P<0.001),FPN(P<0.01)and FTH1(P<0.05)were decreased in HT22 cells.The ferritin-related protein levels,iron levels,cell viability and apoptosis rate of DMSO group were basically the same as those of normal control group.Compared with Erastin group,the cell viability of neurons(P<0.001)and the GPX4 enzyme level of the cells(P<0.01)in the curcumol treatment group was significantly increased,the apoptosis rate(P<0.001)and the total iron and ferrous iron contents of the cells(P<0.001)were decreased oppositely.In addition,the expression levels of Xct(P<0.001),NCOA4(P<0.001),and ACSL4(P<0.001)were significantly down-regulated,the expression levels of FTH1(P<0.01)and GPX4(P<0.001)were significantly up-regulated(P<0.01).Conclusion Curcumol could protect HT22 cells by inhibiting erastin-induced ferroptosis.
