Exploring the Mechanism of Salvia Miltiorrhiza in the Treatment of Hypoxic Pulmonary Hypertension Based on Network Pharmacology and Experimental Validation
- VernacularTitle:基于网络药理学及实验验证探究丹参治疗低氧性肺动脉高压的机制
- Author:
Zhengwei DONG
1
;
Min ZHANG
;
Huan ZHAO
;
Zuoying XING
;
Rui YU
;
Guanwei FAN
;
Yongxia WANG
;
Mingjun ZHU
Author Information
- Keywords: Salvia miltiorrhiza; Network pharmacology; Hypoxic pulmonary hypertension; Pulmonary fibrosis; PI3K/AKT signalling pathway
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(8):2023-2029
- CountryChina
- Language:Chinese
- Abstract: Objective Based on the pre-existing basis of effective treatment of hypoxia combined with Su5416-induced hypoxic pulmonary hypertension(HPH)by Salvia miltiorrhiza,to investigate the mechanism of Salvia miltiorrhiza in the treatment of HPH.Methods Using a network pharmacology approach to obtain the key pathways of Salvia miltiorrhiza for the treatment of HPH.The active ingredients of Salvia miltiorrhiza were collected to obtain the targets of the active ingredients.HPH disease targets were collected to obtain the intersection of Salvia miltiorrhiza component targets and HPH disease targets.Protein-Protein Interaction Networks(PPIs)were constructed and KEGG analysis was performed to obtain the key pathways of Salvia miltiorrhiza for HPH.Then used molecular biology to validate the key pathways.Results The 81 targets of Salvia miltiorrhiza for the treatment of HPH were obtained by network pharmacology,and PPI showed that drug component-disease common core targets included ATK1,TNF,EGFR,IL6,ESR1,and KEGG-enriched Pathway mainly included PI3K-AKT signaling pathway,HIF-1 signaling pathway,MAKP signaling pathway,TNF signaling pathway,JAK-STAT signaling pathway and so on.Molecular biological assays showed that Salvia miltiorrhiza had the effect of reducing lung tissue fibrosis and inhibiting the PI3K/AKT signalling pathway in HySu-PH mice.Conclusion Salvia miltiorrhiza has the effect of attenuating pulmonary fibrosis,and its mechanism of action is related to the inhibition of the PI3K/Akt signalling pathway.
