Determination of Four Compounds and Optimization of Extraction Technology of Total Flavonoids in Pteris multifida Formula Granules
- VernacularTitle:凤尾草配方颗粒4个成分含量同时测定及其总黄酮提取工艺优化
- Author:
Liaoyuan LIU
1
;
Xintong YU
;
Shan WEN
;
Dongting XU
;
Weimei CHEN
;
Chang SHAO
;
Jiangping CHEN
Author Information
- Keywords: Pteris multifida formula granules; Quantitative analysis of multi-compounds by single-marker; Total extraction rate of total flavonoids; Extraction technology; Box-Behnken test
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(7):1819-1829
- CountryChina
- Language:Chinese
- Abstract: Objective To establish a quantitative analysis of multi-compounds by single-marker(QAMS)method for the simultaneous determination of four compounds in formula granules of Pteris multifida and optimize the extraction process of total flavonoids.Methods The relative correction factors(RCFs)of lonicerin,luteolin and apigenin in the formula granules of Pteris multifida were calculated by using the UPLC method with rhoifolin as the internal reference,and their durability was investigated.The external standard method(ESM)was used to determine the content of four compounds in the formula granules of Pteris multifida,and the difference between the calculated values and the measured values was compared.The effects of ultrasonic time,ethanol volume fraction,solid-liquid ratio and ultrasonic power on the extraction rate of total flavonoids were studied by single factor experiment.On this basis,Box-Behnken test with three factors and three levels was conducted to optimize the extraction process of total flavonoids,and the verification experiment was conducted.Results The content of rhoifolin in the formula granules was 0.035%-0.056%,and the content of lonicerin,luteolin and apigenin by QAMS method was 0.085%-0.167%,0.014%-0.028%and 0.004%-0.008%,respectively,which had no significant difference with the external standard method.The optimal extraction conditions of total flavonoids were 80%ethanol water,solid-liquid ratio 1:20,40 minutes extraction,the average extraction rate was 24.46 mg·g-1.Conclusion The established QAMS method was accurate and feasible,and the optimized extraction process of total flavonoids based on Box-Behnken response surface method was simple and feasible,which could lay a foundation for the quality evaluation of the Pteris multifida formula granules.
