To Study the Mechanism of"Yajieshaba"Against Acute Alcoholic Liver Injury in Mice Based on 16S rDNA Technology
- VernacularTitle:基于16S rDNA技术研究"雅解沙把"抗急性酒精性肝损伤小鼠的作用机制
- Author:
Yuanmei BAI
1
;
Feifan LIU
;
Lijie ZHENG
;
Yan WAN
;
Jiachen FAN
;
Jiahao DENG
;
Peixin GUO
;
Qiongchao LI
Author Information
- Keywords: "Yajieshaba"; 16S rDNA; Intestinal flora; Liver injury; Pharmacodynamic efficacy
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(6):1583-1592
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the pharmacological effects of"Yajieshaba"on mice with alcohol-induced liver injury and to investigate the mechanism of the impact of"Yajieshaba"on the regulation of intestinal flora by 16S rDNA technology.Methods Healthy male KM mice were randomly divided into control,model,"Yajieshaba"low,medium,and high dose(0.39,1.17 and 3.51 g·kg-1)groups and Bifendatatum(2.93 mg·kg-1)groups,with eight mice in each group.After one week of pre-administration of"Yajieshaba",a mouse model of acute alcoholic liver injury was established by a single instillation of 56%alcohol,and the levels of AST and ALT in the serum of mice were measured,and the morphological changes of liver histology were observed by HE staining;secondly,faecal DNA was extracted from each group under aseptic operation,and 16S rDNA sequencing and differential analysis by alpha diversity and species composition at the phylum and genus levels were performed.Results The results showed that the biochemical indexes of liver function(ALT and AST)were significantly improved by"Yajieshaba",and the degree of liver damage was significantly reduced by HE staining.At the phylum level,it significantly decreased the abundance of Aspergillus and increased the quantity of Bacteroides;at the genus level,it significantly up-regulated the plenty of Bacteroides and Prevotella and downregulated a lot of Prevotella and Helicobacter.At the genus level,"Yajieshaba"significantly up-regulated the abundance of Bacillus spp.and Prevotella spp.and down-regulated the abundance of Prevotella spp.and Helicobacter spp.Conclusion"Yajieshaba"may play an anti-acute alcoholic liver injury effect by regulating the intestinal flora and metabolites.
