Clinical value of a rapid respiratory syncytial virus antigen detection in point-of-care testing
- VernacularTitle:人呼吸道合胞病毒快速抗原检测方法的床旁检测适用性探讨
- Author:
Yaxin DING
1
;
Run TIAN
;
Yuan QIAN
;
Yu SUN
;
Jie DENG
;
Fang WANG
;
Runan ZHU
;
Linqing ZHAO
Author Information
1. 首都儿科研究所病毒研究室 儿童病毒病病原学北京市重点实验室
- Keywords:
Respiratory syncytial virus;
Point-of-care testing;
Antigen detection
- From:
Chinese Journal of Pediatrics
2017;55(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical value of a rapid respiratory syncytial virus ( RSV ) antigen detection in point-of-care testing (POCT).Method A total of 209 specimens, including 78 throat swabs (TS) and 131 nasopharyngeal aspirates ( NPAs), were collected from inpatients who visited the Children′s Hospital Affiliated to the Capital Institute of Pediatrics and were diagnosed as acute respiratory infection from 5 January to 7 February , 2015.These specimens were tested for RSV by a rapid antigen detection kit which was compared with reverse transcription polymerase chain reaction ( RT-PCR) and direct immunofluorescence assay ( DFA ) for RSV detection. Result Compared with DFA for NPAs , the sensitivity and specificity of rapid antigen detection were 83.9% and 97.3%, respectively , with Kappa value of 0.86; Compared with RT-PCR, the sensitivity ( NPAs, 74.2%; TS, 77.8%) and specificity ( NPAs, 100.0%;TS, 92.0%) of rapid antigen detection were high , too, with Kappa value of 0.74 in NPAs and 0.62 in TS.However , the RSV positive rate of rapid antigen detection in TS ( 21.7%) from pediatric patients with acute lower respiratory tract infection was lower than that in NPAs (78.3%), as well as that of RT-PCR (7.3%in TS verse 78%in NPAs).The RSV rapid antigen detection kit can be finished in about 10 minutes.Conclusion With characteristics of high specificity , high sensitivity , being rapid , efficient and easy to operate in comparison with DFA and RT-PCR, RSV rapid antigen detection in this study is suitable for POCT.For pediatric patients with acute respiratory tract infection , NPA was better than TS for RSV detection.
