Investigating Interventional Mechanism of Bufei Yishen Formula for Inhibiting Macrophage Inflammatory Response Based on Gene Set Enrichment Analysis
- VernacularTitle:基于基因集富集分析阐释补肺益肾方抑制巨噬细胞炎症反应机制
- Author:
Qingzhou GUAN
1
,
2
;
Peng ZHAO
;
Yange TIAN
;
Kangchen LI
;
Yan PENG
;
Hongyan GUO
;
Jiansheng LI
Author Information
- Keywords: Chronic obstructive pulmonary disease; Bufei Yishen formula; Macrophage; Inflammation; Gene set enrichment analysis
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(6):1490-1496
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the interventional mechanism of Bufei Yishen formula on chronic obstructive pulmonary disease from pathway level.Methods Macrophage inflammatory model was established by LPS stimulation.Based on gene set enrichment analysis(GSEA)method,macrophage inflammation related pathways were screened,and normalized enrichment score(NES)was used to identify pathways that were reversed by traditional Chinese medicine treatment,revealing the interventional mechanism of Bufei Yishen formula and its compatibility.Results The NES of Bufei Yishen formula was-1377.23,among which the NES of Bushen compatibility was-485.07,that of Huoxue was-351.86,Huatan was-303.71,and Yiqi was-236.59.There were 213 significantly disturbed pathways reversed after the intervention of Bufei Yishen formula,among which there were 184 reversed pathways for Huoxue compatibility,147 reversed pathways for Bushen,134 reversed pathways for Huatan,133 reversed pathways for Yiqi,and the reversal rate was 75.41%,60.25%,54.92%,54.51%,respectively.90 pathways,including TGF-β production,were significantly reversed in the four compatibilities.Positive regulation of cytokine production involved in inflammatory response etc were specifically reversed pathways for compatibility.Conclusion The intensity of Bufei Yishen formula that reversed macrophage-inflammatory related pathways was in order of Bushen,Huoxue,Huatan and Yiqi compatibility.And the number of pathways that could be reversed by the compatibility of Bufei Yishen formula was Huoxue,Bushen,Huatan and Yiqi.Bufei Yishen formula could regulate the common and specific reversal pathways of the compatibilities to intervene the inflammatory response.
