Study on the Prevention and Treatment of Postoperative Abdominal Adhesion by Inhibiting Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells with Supplemented Buyang Huanwu Decoction
- VernacularTitle:加味补阳还五汤抑制腹膜间皮细胞上皮-间充质转换防治术后腹腔粘连的实验研究
- Author:
Minlin ZHENG
1
;
Wenjiang FAN
;
Yanan WANG
;
Qianqian ZHAN
Author Information
- Keywords: Postoperative abdominal adhesions(PAA); Supplemented Buyang Huanwu Decoction; Epithelial-mesenchymal transition(EMT); E-cadherin; α-smooth muscle actin
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(6):1458-1470
- CountryChina
- Language:Chinese
- Abstract: Objective To evaluated the efficacy of Supplemented Buyang Huanwu Decoction in preventing and treating postoperative abdominal adhesions.Methods One hundred and eight male SD rats were randomly divided into ordinary group,mold group,Sodium hyaluronate group and Supplemented Buyang Huanwu Decoction group.The animals were sacrificed 7,14 and 28 days after the operation,Visual scoring was performed,and samples were taken from the injury site for hematoxylin-eosin staining and Masson staining,and scanning electron microscope to observe the morphology of peritoneal mesothelial cells in the modeling site.Immunohistochemical method was used to observe the expression of epithelial mesenchymal transition(EMT)related cell marker proteins E-cadherin and α-SMA in the adhesive site.Results ①Efficacy of Supplemented Buyang Huanwu Decoction in the prevention and treatment of postoperative abdominal adhesions:The Supplemented Buyang Huanwu Decoction group can reduce the Diamod visual adhesion score(P<0.05),as well as the adhesion levels of HE staining and Masson staining(P<0.05).②Degree of damage to peritoneal mesothelial cells:Scanning electron microscopy showed that in the field of view of the Supplemented Buyang Huanwu Decoction group,paving like peritoneal mesothelial cells could be seen,indicating that the Supplemented Buyang Huanwu Decoction can improve the damage of peritoneal mesothelial cells on the serosal side of the cecum in PAA.③Expression of Epithelial mesenchymal transition-related indicators:The expression of E-Cadherin protein in the Supplemented BuyangHuanwu Decoction group increased(P<0.05).The expression of α-SMA protein in the Supplemented Buyang Huanwu Decoction group decreased(P<0.05).Conclusion The effect of Supplemented Buyang Huanwu Decoction in the prevention and treatment of postoperative abdominal adhesions is exact,which mechanism may be by reducing Epithelial mesenchymal transition of peritoneal mesothelial cells,reducing the deposition of extracellular matrix,thereby reducing peritoneal adhesion.
