Protective Effect of Buxue Shengsui Recipe on Cancer Related Anemia and Immunohypofunction Based on the Zebrafish Model
10.11842/wst.20230628002
- VernacularTitle:基于斑马鱼模型的补血生髓方对肿瘤相关性贫血和免疫功能低下的保护作用
- Author:
Shuo ZHANG
1
;
Xiaoyan JIANG
;
Yingjun FU
;
Honghui NI
;
Shuiying YANG
;
Yu WU
;
Min PENG
Author Information
1. 南通市中医院 南通 226001
- Keywords:
Buxue Shengsui Recipe;
Anemia;
Immune injury;
Zebrafish;
Erythrocyte;
Neutrophils
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2024;26(5):1320-1327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Buxue Shengsui Recipe on anemia and immune injury in zebrafish and its mechanism.Methods Tg(gata1a:DsRed)zebrafish was used as experimental subjects to establish phenylhydrazine-induced zebrafish anemia model.To observe the effect of different concentrations of Buxue Shengsui Recipe on the average strength of zebrafish dorsal blood vessels.Besides,Tg(lysc:DsRed)zebrafish was used as experimental subjects to establish immune injury model of zebrafish induced by chloramphenicol and vinorelbine.To observe the effect of Buxue Shengsui Recipe on the number of neutrophils in zebrafish tail.The expression of interleukin 6(IL-6),interleukin 10(IL-10)and tumor necrosis factor α(TNF-α)mRNA was detected by Real time-PCR.Results Compared with the model group,0.5 mg·mL-1 and 2 mg·mL-1 Buxue Shengsui Recipe significantly increased the average strength of zebrafish dorsal blood vessels(P<0.01)in a dose-dependent manner.2 mg·mL-1 Buxue Shengsui Recipe significantly reversed the decrease of neutrophils caused by chloramphenicol and vinorelbine(P<0.01).Compared with the model group,the expression of IL-6 and IL-10 mRNA of zebrafish in Buxue Shengsui formula group was significantly increased(P<0.05 or P<0.01),and TNF-α mRNA expression was significantly decreased(P<0.05).Conclusion Buxue Shengsui Recipe can improve the anemia state of zebrafish induced by phenylhydrazine,and has obvious protective effect on the immune injury of zebrafish induced by chloramphenicol and vinorelbine,which is related to the up-regulation of cytokines IL-6,IL-10 and the down-regulation of TNF-α.
