Endophytes of Nanyang Mugwort(Artemisia argyi)and Correlation Between Key Communities and Secondary Metabolites
- VernacularTitle:南阳艾内生菌与关键次生代谢产物的相关性探究
- Author:
Mengzhi LI
1
;
Chao LI
;
Zhanhu CUI
;
Yan WEI
;
Lin ZHU
;
Xianzhang HUANG
Author Information
- Keywords: Nanyang Mugwort; Endophytes; Diversity; Composition; Secondary metabolites
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(5):1202-1212
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the effect of endophytes on Nanyang Mugwort by analyzing the diversity of endophytic flora and its correlation with the content of key secondary metabolites in three compartments of Nanyang Mugwort,and the effect of medicinal material quality.Methods In this study,high-throughput sequencing and high-performance liquid chromatography(HPLC)were carried out to determine the distribution endophytes and key secondary metabolites in three compartments of Nanyang Mugwort respectively.The endophytic communities that were significantly correlated with key secondary metabolites were screened by Pearson correlation analysis.Results We found endophytic diversity and composition showed compartment specificity,and bacterial and fungal alpha diversity values(Chao and Ace)in the root compartment were significantly higher than that of leaf and stem.Linear discriminant analysis efect size(LEfSe)analyses identified some potential microbial biomarkers,such as Rhizobium,Streptomyce,Xanthomonadaceae,and Sordariomycetes.Pearson correlation analysis showed that endophytes(such as Rhizobium,Pseudomonas,Flavobacterium,Streptomyces Aspergillus,and Olpidium)were significantly correlated with contents of some phenylpropanoid and flavonoid metabolites in our study(P<0.05).Conclusion In this study,some endophytic communities with significant correlation between key secondary metabolites of Nanyang Mugwort were identified,which provided valuable information to guide the isolation of endophytic strains related to key secondary metabolites and improve the quality of Nanyang Mugwort.
