Determination of Biological Titers of Ranae Oviductus Anti-Aging Vitality
- VernacularTitle:哈蟆油抗衰老活力生物效价检测方法的研究
- Author:
Jiyuan JIA
1
,
2
,
3
;
Dacheng JIANG
;
Zhen ZHANG
;
Yunlong SUN
Author Information
- Keywords: Ranae Oviductus; Quality standard; Biological titer; Anti-aging vitality
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(4):1008-1016
- CountryChina
- Language:Chinese
- Abstract: Objective Based on the efficacy of Ranae Oviductus in reinforcing the kidney and replenishing essence,a biological titer assay for Ranae Oviductus was developed using anti-ageing vitality as an indicator,and this was used to evaluate the quality of Ranae Oviductus from different origins and to provide a reference for improving its quality standard.Methods MRC-5 cells were used as the study target and H2O2 was used as the acute senescence model-inducing drug to verify the anti-aging effect of Ranae Oviductus by cell viability and β-galactosidase staining rate.Vc was selected as the reference and CCK-8 method was used as the biological titer assay for Ranae Oviductus.Results In the cell viability assay,compared to the model group,the Ranae Oviductus group was able to up-regulate cell viability,with the best effect at 250.0 μg·mL-1,up-regulating by 20.01％.The percentage of SA-β-gal positive cells was reduced by 17.70％in the cells pretreated with Ranae Oviductus.The results of the biological titer of anti-aging determination showed that the biological titer of anti-aging of Ranae Oviductus from different origins ranged from 218.03 to 1152.04 U·mg-1,with an extreme difference of 934.01 U·mg-1.Conclusion The established biological titer assay for Ranae Oviductus can clearly distinguish the differences in the quality of Ranae Oviductus and provide a certain reference for the quality evaluation and control of Ranae Oviductus.
