Integrating LC-MS and Network Pharmacology Analysis to Explore the Mechanism of Yishenqingli Formula in Treating Idiopathic Membranous Nephropathy
10.11842/wst.20230305002
- VernacularTitle:联合液相色谱-质谱分析和网络药理学探讨益肾清利方治疗特发性膜性肾病的作用机制
- Author:
Neng BAO
1
;
Xiang YU
;
Mingjia GU
;
Jin WANG
;
Xin GU
;
Yafen YU
;
Wei KONG
Author Information
1. 江南大学附属医院 无锡 214000
- Keywords:
Yishenqingli formula;
Idiopathic membranous nephropathy;
Liquid chromatography-mass spectrometry;
Network pharmacology;
Molecular docking
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2024;26(3):797-807
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to explore the potential targets of Yishenqingli Formula in treating idiopathic membranous nephropathy(IMN)using a combination of liquid chromatography-mass spectrometry(LC-MS)analysis and network pharmacology.Methods The active ingredients of the Yishen Qingli Formula were identified through the BATMAN-TCM database and LC-MS qualitative analysis.The biological processes and mechanism pathways of the Yishen Qingli Formula in treating IMN were predicted using network pharmacology,and molecular docking and in vitro,experiments were conducted to verify the selected core targets.The core targets were selected and validated through molecular docking and in vitro experiments.Results A total of 15 active ingredients were selected from the Yishen Qingli Formula,and 72 core genes were obtained by intersecting its target with the IMN disease target.GO enrichment analysis results showed that the regulation of apoptosis signaling pathway,white cell migration,peptide tyrosine phosphorylation,and so on were involved;The KEGG pathway enrichment analysis results showed that the treatment of IMN with Yishen Qingli Formula involves apoptosis-related signaling pathways such as TNF,PI3K/AKT,MAPK,etc.In vitro,experiments have shown that Yishen Qingli Formula can reduce podocyte apoptosis by regulating the PI3K/AKT pathway.Conclusion Yishen Qingli Formula is a treatment for idiopathic membranous nephropathy through multiple targets and pathways.It has an anti-apoptotic effect on the C5b-9 induced podocyte sub-lysis model,and its mechanism of action may be related to the TNF,PI3K/AKT,MAPK signaling pathways.
