Exploring the mechanism of curcumin in the treatment of post-stroke depression based on network pharmacology and molecular docking
10.16557/j.cnki.1000-7547.2024.04.009
- VernacularTitle:基于网络药理学及分子对接探讨姜黄素治疗卒中后抑郁的作用机制
- Author:
Jianxiang WANG
1
;
Xiaojiao YIN
;
Yun GAO
Author Information
1. 昆明医科大学附属甘美医院,昆明 650000
- Keywords:
curcumin;
ischemic stroke(IS);
depression;
post-stroke depression(PSD);
network pharmacology;
molecular docking
- From:
Chinese Journal of Neuroanatomy
2024;40(4):464-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential molecular mechanism of curcumin in the treatment of post-stroke depression through network pharmacology and molecular docking.Methods:The potential targets of curcumin were searched in GeneCards,PharmGKB and SwissTargetPrediction database.The online Mendelian Inheritance in Man data-base and GeneCards database were searched for potential targets of ischemic stroke and depression.The targets were standardized by Uniprot and then imported into Venn online platform to obtain the intersection targets among the three.Protein-protein interaction(PPI)was visualized by STRING and Cytoscape 3.7.1 software.Metascape database was used to perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of intersection targets.Finally,AutoDock Vina1.5.6 software was used to verify the molecular docking between curcumin and core targets.Results:A total of 1452 potential targets for ischemic stroke and depression,207 drug tar-gets,and 47 intersection targets of the three were obtained.GO functional enrichment analysis yielded 2332 items,and KEGG pathway enrichment analysis yielded 138 items.According to the degree value and enrichment fraction,the core targets such as interleukin-6(IL-6),tumor necrosis factor(TNF),serine/threonine protein kinase 1(AKT1),inter-leukin-1β(IL-1β)and the advanced glycation end products receptor(AGE-RAGE)signaling pathway,interleukin-17(IL-17)signaling pathway,TNF signaling pathway,etc.Molecular docking results showed that curcumin had a strong binding ability to core targets such as IL-6,TNF,AKT1,and 1L-1β.Conclusion:Curcumin may act on key targets such as IL-6,TNF,AKT1,IL-1β,and play an important role through the AGE-RAGE,IL-17,and TNF signaling pathways.
