Establishment of a post-stroke dysphagia mouse model by photothrombosis method
10.16557/j.cnki.1000-7547.2024.04.007
- VernacularTitle:光栓塞法建立脑卒中后吞咽障碍小鼠模型
- Author:
Cong TIAN
1
;
Zehua RAO
;
Tong RAO
;
Meng LU
;
Ankun CHEN
;
Xin LIU
;
Zhimiao MURONG
;
Zenghui YUE
Author Information
1. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Keywords:
photothrombosis method;
post-stroke dysphagia(PSD);
dysphagia;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(4):452-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a feasible mouse model of post-stroke dysphagia(PSD).Methods:Thirty C57BL/6 male mice were randomly divided into a sham-operated group(Sham)and a model group(PSD),and the PSD mouse model was made by the photothrombosis method(PT)method,and the sham-operated group was only injec-ted with rose bengal staining solution in the tail vein.The cerebral blood flow of the mice was measured by laser scatter imaging,the ratio of cerebral infarct area was detected by TTC staining,the electromyographic area of the in vivo pha-ryngeal muscle group of mice swallowing was recorded by a multi-conductor physiological recorder MP160,the drinking function of the mice was measured by the 4-min water drinking experiment,and the weight changes were recorded,respectively,at 1,3,and 7 d.Results:Cerebral blood flow decreased at all time points,with a sharp drop in cerebral blood flow at 1 d,gradual recovery of cerebral blood flowat 3 and 7 d,establishment of collateral circulation,and gradu-al reduction of cerebral infarction area;compared with the Sham group,the myoelectric area of the PSD group was reduced at 1 and 3 d(P＜0.05),but with a trend of gradual recovery,and there was no significant difference between the PSD group and the Sham group at 7 d,and water consumption and weight decreased at 4 min at 1,3,and 7 d(P＜0.05).Conclusion:The mice showed some degree of dysphagia symptoms and are expected to be a translational model for PSD.
