Mdivi-1 improves motor function of mice with hepatic encephalopathy by repairing mitochondrion in substantia nigra reticulata
10.16557/j.cnki.1000-7547.2024.04.002
- VernacularTitle:Mdivi-1通过修复黑质网状部线粒体改善肝性脑病小鼠运动功能
- Author:
Jingjing TIE
1
;
Xiaodong LI
;
Ziwei NI
;
Xin HUANG
;
Feifei WU
;
Lu WANG
;
Yanling YANG
;
Yayun WANG
Author Information
1. 延安大学医学院人体解剖与组织胚胎学教研室,延安 716000
- Keywords:
acute hepatic encephalopathy(AHE);
substantia nigra pars reticulata(SNr);
mitochondrion;
Mdivi-1;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(4):413-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of mitochondria in the substantia nigra pars reticulata(SNr)in a mouse model of acute hepatic encephalopathy(AHE),and the effects of mitochondrial division inhibitor Mdivi-1 on the motor function and mitochondrial function of SNr in AHE mice.Methods:The mouse model of AHE was established by intraperitoneal injection of thioacetamide(TAA)and treated with Mdivi-1.The changes of serum aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT),and blood ammonia were detected by biochemical detection kits.Open field test,rotor-rod fatigue test and elevated plus maze test were performed to observe the motor function of AHE mice.Mitochondrial membrane potential(MMP),cellular reactive oxygen species(ROS)and ATP of SNr were detected by commercial kits.Results:Compared with the control group,the levels of AST,ALT and blood ammonia in AHE mice were increased.The total movement distance of the mice in the open field was reduced,and the movement time of the rotor-rod fatigue test and the elevated plus maze test were shortened.In SNr,mitochondria became smaller and rounder,mitochondrial fission increased,MMP decreased,cellular ROS increased,and ATP production decreased.After treat-ment with Mdivi-1,the levels of AST,ALT and blood ammonia in AHE mice were decreased.In the open field,the total movement distance of mice increased,the movement time of rotorrod fatigue test and elevated plus maze test increased,the mitochondria of SNr were larger,with decreased roundness,decreased mitochondrial division,increased MMP,decreased cellular ROS,and increased ATP production.Conclusion:Mdivi-1 can improve movement disorders in AHE mice by repairing mitochondrial in the SNr.
