Morphological study on projections of tyrosine hydroxylase positive neurons in locus coeruleus to paraventricular nucleus of the thalamus in mice
10.16557/j.cnki.1000-7547.2024.04.001
- VernacularTitle:小鼠蓝斑内酪氨酸羟化酶阳性神经元向丘脑室旁核投射的形态学研究
- Author:
Pengxin ZHANG
1
,
2
;
Hui ZHU
;
Fei PENG
;
Peiyuan LYU
;
Huijie FENG
;
Meiqi XUE
;
Yijia XUE
;
Yulin DONG
Author Information
1. 空军军医大学基础医学院人体解剖学教研室暨梁銶琚脑研究中心,西安 710032
2. 空军军医大学基础医学院学员四大队,西安 710032
- Keywords:
locus coeruleus(LC);
paraventricular nucleus of thalamus(PVT);
tyrosine hydroxylase(TH);
tachykinin-1(TAC1);
nociceptive information;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(4):405-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the projections from tyrosine hydroxylase(TH)positive neurons in locus coerule-us(LC)to tachykinin-1(TAC1)neurons in paraventricular nucleus of the thalamus(PVT),and morphologically determine whether they are involved in transmission and modulation of nociceptive information.Methods:TAC1-ires-Cre mice were hybridized with Rosa26:CAG-LSL-tdTomato(Ai9)mice.And spared nerve injury(SNI)induced neu-ropathic pain model was established with TAC1-ires-Cre::Ai9 mice to observe the colocalization of TAC1 and Fos and the close appositions between TAC1/FOS double-labeled neurons and TH positive axonal terminals.The distribution of the TH positive neurons and FG retrogradely labeled neurons were observed in the LC after Fluorogold(FG)was injec-ted into the PVT.Finally,the coexistences of TH positive neurons and RV labeled neurons in the LC were observed after injection of RV-mediated retrograde tracing system.Results:TAC1 positive neurons were shown with red fluores-cence in TAC1-ires-Cre::Ai9 mice.TAC1/FOS double-labeled neurons were found in the PVT of the SNI model.Some TAC1/FOS double labeled neurons made close appositions with TH positive axonal terminals.FG retrogradely labeled neurons were observed in the LC after FG injected into the PVT,and some of the FG labeled neurons coexisted with TH positive neurons.Using RV retrograde transsynaptic tracing virus,the results showed that presynaptic neurons of TAC1 positive neurons in the PVT were found in the LC,and most of the presynaptic neurons were TH positive neu-rons.Conclusion:TH positive neurons in the LC project to TAC1 positive neurons of the PVT,forming LCTH+-PVTTAC1+neural circuit,which were activated by nociceptive information.It demonstrates that this pathway plays a role in pain transmission or regulation.
