Osteopontin inhibits M1 polarization of microglia in rats with subarachnoid hemorrhage
10.16557/j.cnki.1000-7547.2024.03.014
- VernacularTitle:骨桥蛋白抑制蛛网膜下腔出血大鼠的小胶质细胞向M1极化
- Author:
Ligang CHEN
1
;
Zheng ZOU
;
Guangzhi HAO
;
Yushu DONG
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Keywords:
osteopontin(OPN);
subarachnoid hemorrhage(SAH);
early brain injury(EBI);
microglia;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(3):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Osteopontin(OPN)has demonstrated neuroprotective effects in various stroke models.Its role in neuroinflammation after brain injury remains to be elucidated.This study aims to clarify the effect of OPN on neuroin-flammation,particularly on the functional states of microglia after subarachnoid hemorrhage(SAH).Methods:Thirty rats were randomly divided into the following groups:Sham,SAH,and SAH+OPN.SAH rat model was prepared by secondary injection of autologous arterial blood,and OPN was given intranasally in the treatment group.Neurological function was evaluated by modified Garcia score.The degree of cerebral edema was evaluated by measuring brain water content.The expression of microglia activation markers CD86,inducable nitric oxide synthase(iNOS),CD206 and arginase 1(Arg-1)after SAH and OPN treatment was detected by RT-qPCR.The levels of IL-1β,IL-6,IL-10,and IL-13 in cerebrospinal fluid were detected by enzyme-linked immunosorbent assay(ELISA).Results:Intranasal ad-ministration of OPN could improve the neurological dysfunction and cerebral edema in SAH rats.What's more,OPN could inhibit the expression of CD86,iNOS,IL-1β,and IL-6 in cerebral while promote the expression of CD206,Arg-1,IL-10,and IL-13.Conclusion:OPN alleviates the inflammatory response after SAH by inhibiting the polarization of microglia M1.
