Gastrodin inhibits the lipopolysaccharide-induced microglia activation through the SOX2/β-catenin signaling pathway
10.16557/j.cnki.1000-7547.2024.03.013
- VernacularTitle:天麻素通过SOX2/β-catenin通路抑制脂多糖诱导的小胶质细胞活化
- Author:
Xinglin ZHANG
1
;
Jinsha SHI
;
Hongming FU
;
Yurui HUANG
;
Haolong SHI
;
Hanjun ZUO
;
Tao GUO
;
Juanjuan LI
Author Information
1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
- Keywords:
gastrodin(GAS);
lipopolysaccharide(LPS);
sex-determining region Y-box2(SOX2);
β-catenin;
microglia
- From:
Chinese Journal of Neuroanatomy
2024;40(3):365-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of gastrodin(GAS)on the sex-determining region Y-box2(SOX2)/β-catenin pathway in microglia induced by lipopolysaccharide(LPS).Methods:BV2 microglia was cultured in vitro and divided into the following groups:Control group(Control),LPS group(LPS),LPS+0.17 mmol/L gastrodin treatment group(LPS+GAS-L),LPS+0.34 mmol/L gastrodin treatment group(LPS+GAS-H),SOX2 inhibitor pronethalolgroup(PR),LPS+PR group(LPS+PR),and LPS+PR+GAS group(LPS+PR+GAS).Effect of PR on BV2 microglia viability was detected by CCK-8.The expression of SOX2,β-catenin,mannose receptor(CD206)and tumor necrosis factor-α(TNF-α)was assessed using Western Blot and immunofluorescence double staining.Results:PR did not induce significant BV2 cell death in the 0～40 μmol/L range.After LPS treatment,the expression levels of SOX2,β-catenin,and TNF-α significantly increased in the LPS group,while CD206 decreased(P＜0.05).Following GAS treatment,the expression levels of SOX2,β-catenin,and TNF-α significantly decreased,while CD206 increased(P＜0.05).Compared to the LPS group,the expression levels of β-catenin and TNF-α significantly de-creased in the PR group(P＜0.05),but no significant difference was observed between the LPS+GAS and LPS+PR+GAS group.Conclusion:GAS significantly inhibits LPS-induced microglia activation potentially through the inhibi-tion of the SOX2/β-catenin signaling pathway,and exerts anti-inflammatory effects.
