Transcriptomic analysis of delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage
10.16557/j.cnki.1000-7547.2024.03.009
- VernacularTitle:动脉瘤性蛛网膜下腔出血后迟发性脑缺血的转录组学分析
- Author:
Guangzhi HAO
1
;
Yuwei HAN
;
Da HUO
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Keywords:
gene expression database;
aneurysmal subarachnoid hemorrhage(aSAH);
delayed cerebral ischemia(DCI);
transcriptomic analysis
- From:
Chinese Journal of Neuroanatomy
2024;40(3):341-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the key differentially expressed genes and related pathways in patients with de-layed cerebral ischemia(DCI)occurring after aneurysmal subarachnoid hemorrhage(aSAH)based on gene expression database(GEO)datasets.Methods:Gene datasets meeting the study requirements were screened from the GEO data-base and divided into a patient group with DCI after aSAH and a control group without DCI,and differentially expressed genes(DEGs)analysis,gene ontology(GO)enrichment analysis,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,and gene set enrichment analysis(GSEA)were performed by using R software to find rele-vant genes and pathways.Results:A total 140 differentially expressed genes were identified.KEGG analysis showed that there were 10 significantly enriched signaling pathways(P＜0.05),mainly related to hypoxia-inducible factor-1(HIF-1)signaling pathway,glutamatergic synapses,cell adhesion molecules,and chemokine signaling pathways,etc.Twenty-six significantly enriched entries were obtained according to the functional analysis of GO,which involved entries in three categories,namely,biological processes,cellular components,and molecular functions.GSEA analysis showed that two pathways,extracellular matrix receptor interactions as well as peroxisomes,had significant enrichment differ-ences between the patient group and the control group.Conclusion:The development of DCI after aSAH involves sever-al genes such as COL17A1,RPL11,FCAMR,GNB2L1,HIF1A,and can affect the development of DCI through various pathways such as vascular dysfunction,inflammatory response,neurological injury,and oxidative stress.
