Lipopolysaccharide was administered via a self-made cannula in the cerebellomedullary cistern of rats to induce CNS infection
10.16557/j.cnki.1000-7547.2024.03.008
- VernacularTitle:通过自制套管给予小脑延髓池脂多糖诱发大鼠中枢神经系统感染
- Author:
Yao GUO
1
;
Chang GUO
;
Kaini WANG
;
Ruoqi ZANG
;
Jie GAO
;
Yang MA
;
Yitong CHEN
;
Yu ZHANG
Author Information
1. 山西医科大学口腔医学院,太原 030001
- Keywords:
cerebellomedullary cistern(CMC);
caudal spinal trigeminal nucleus(SpVc);
oralfacial pain;
catheter administration;
neuroinflammation;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(3):334-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study introduces a novel approach utilizing a self-made drug delivery cannula implanted into the cerebellomedullary cistern(CMC)of rats to allow repeated administrations in conscious subjects.Methods:A self-made medication cannula is inserted through a drilled hole at the midpoint of the occipital crest of the rat's skull,de-scending along the inner wall of the occipital bone until reaching the CMC,and securing it in place with skull screws and self-curing resin.Lipopolysaccharide(LPS)is injected into the CMC to induce neuroinflammation,and the feasibility of this method is assessed using X-ray imaging,behavioral testing,and immunofluorescence staining.Results:The place-ment of the brain cannula was confirmed using X-ray film and pontamine sky blue staining.Rats in the LPS group exhib-ited a lower facial mechanical pain threshold compared to the Control group(P＜0.001),along with reduced residence time in the open field center(P＜0.01).Immunofluorescence staining revealed LPS-induced activation of caudal spinal trigeminal nucleus(SpVc)microglia.Conclusion:This method proves to be suitable for multiple administrations to the cerebellomedullary cistern of conscious rats,enabling the study of the SpVc's role in pain modulation.
