L-arginine and choline chloride improve the learning and memory abilities of Aβ1-42-induced dementia rats
10.16557/j.cnki.1000-7547.2024.03.007
- VernacularTitle:左旋精氨酸和氯化胆碱改善Aβ1-42诱导的痴呆大鼠学习记忆能力
- Author:
Lixia LIU
1
;
Haoyu WANG
;
Wenxiu QI
Author Information
1. 山西医科大学汾阳学院,汾阳 032200
- Keywords:
Alzheimer's disease(AD);
neuronal nitric oxide synthetase(nNOS);
α7 nicotinic acetylcholine receptor(α7nAChR);
Y-maze;
learning and memory;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(3):327-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression changes of neuronal nitric oxide synthetase(nNOS)and α7 nic-otinic acetylcholine receptor(α7nAChR)in prefrontal cortex and hippocampus and the effects on the behavioral func-tions of Aβ induced dementia rats.Methods:Forty adult male Sprague-Dawley(SD)rats were intracerebroventricular-ly(i.c.v.)injected with condensed-amyloid beta peptides 1-42 to establish dementia model.Two drug treatment groups were respectively i.c.v.injected with the nitric oxide(NO)precursor L-arginine(L-Arg)and α7nAChR agonist choline chloride(CC),and another group were injected with them both.Spatial learning and memory functions of rats were evaluated by Y-maze experiment.The expressions of nNOS and α7nAChR in prefrontal cortex and hippocampus were measured by immunohistochemical staining and Western Blot.Results:The results showed that,in comparison with Aβ+NS group,rats in Aβ+L-Arg group or Aβ+CC group exhibited decreases in the numbers to reach the criteri-on(P＜0.05 or P＜0.01),with significant increases of the protein expressions of nNOS and a7nAChR in prefrontal cortex and hippocampus(P＜0.05 or P＜0.01).However,compared with Aβ+L-Arg group or Aβ+CC group,the protein expressions of nNOS and α7nAChR in prefrontal cortex and hippocampus of rats were significantly increased(P＜0.05 or P＜0.01),and the numbers to reach the criterion were decreased in Aβ+L-Arg+CC group(P＜0.05 or P＜0.01).Conclusion:The combined use of L-Arg and CC in the lateral ventricle can significantly enhance the effects of either on the up-regulation of nNOS and α7nAChR,and the improvement of the cognitive impairment in rats.There-fore,it is speculated that the synergistic effect of central nNOS and nicotine system is more beneficial to improving cog-nitive impairment function in dementia rats.
