The effects of scutellarin on P13K expression and phosphorylation level in activated microglia
10.16557/j.cnki.1000-7547.2024.03.006
- VernacularTitle:灯盏乙素对激活的小胶质细胞中PI3K表达和磷酸化水平的影响
- Author:
Lifang YANG
1
;
Zhaoda DUAN
;
Yujia YANG
;
Dongyao XU
;
Chunyun WU
;
Li YANG
Author Information
1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
- Keywords:
cerebral ischemia;
scutellarin;
microglia;
phosphatidylinositol 3-kinase(PI3K);
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(3):320-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the impact of scutellarin on the levels of phosphatidylinositol 3-kinase(P13K)and phosphorylated P13K(p-PI3K)in activated microglia.Methods:A rat cerebral ischemia model was made by middle cerebral artery occlusion(MCAO)method.SD rats were randomly divided into three groups:sham operation group(Sham),cerebral ischemia group(MCAO),and cerebral ischemia with scutellarin group(MCAO+S).A model of ischemia-hypoxia injury of BV2 microglia was established by glucose-oxygen deprivation(OGD)and randomly assigned as control group(Control),OGD group and OGD+scutellarin group(OGD+S).Changes in PI3K and p-PI3K expres-sion in microglia were assessed using double immunofluorescence staining and Western Blot.Results:Immunofluores-cence staining and Western Blot analyses revealed a significant increase in p-PI3K levels in activated microglia both in vivo and in vitro(P＜0.05).Furthermore,treatment with scutellarin led to a further elevation in p-PI3K(P＜0.05).However,there were no significant alterations observed in PI3 K expression among the groups(P＞0.05).Conclusion:Scutellarin may play a positive role in the treatment of cerebral ischemia by up-regulating the phosphorylation level of P13K in activated microglia.
