Mechanism of diosmin in alleviating anxiety of PTSD-like mice based on network pharmacology
10.16557/j.cnki.1000-7547.2024.03.004
- VernacularTitle:基于网络药理学研究地奥司明缓解PTSD小鼠焦虑的分子机制
- Author:
Xinchao ZHANG
1
,
2
;
Hongyu LI
;
Mingmei WU
Author Information
1. 西北大学生命科学学院,西安 710069
2. 空军军医大学基础医学院神经生物学教研室,西安 710032
- Keywords:
diosmin;
post-traumatic stress disorder;
network pharmacology;
animal ethology;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(3):305-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Investigate the potential of diosmin(DSM)in alleviating anxiety behavior associated with post-traumatic stress disorder(PTSD)in mice.Methods:A PTSD-like mouse model was induced with continuous restraint,forced swimming,anesthesia,and electric shock.DSM was administered via intraperitoneal injection.The impact of DSM on anxiety behavior was assessed using open-field and elevated maze tests.Databases such as Swiss Tar-get Prediction,Drug Bank,TTD,and Gene Cards were utilized to gather the pertinent targets associated with DSM and PTSD.The Venny2.1 tool was employed to identify overlapping targets between DSM components and those relevant to PTSD.A protein-protein interaction(PPI)network was constructed and topological analysis was conducted to identify core targets.The core targets were further analyzed through GO classification and KEGG pathway enrichment.The"component-disease-target-pathway"network of DSM anti-PTSD was constructed.Immunofluorescence staining of mouse brain tissue was used to verify the core targets.Results:DSM significantly alleviates anxiety-like behavior in PTSD-like mice.The result of network pharmacology revealed 53 common targets,15 key targets,649 biological proces-ses,and 46 differential signaling pathways of DSM in the treatment of PTSD.Key targets CCL5,JNK,and TNF-α with relatively high screening values were used for immunofluorescence staining of mouse brain slices.The expression of CCL5,JNK,and TNF-α were highly higher in the PTSD group than the normal group,and DSM could significantly in-hibit their expression in PTSD-Like mice.Conclusion:DSM can significantly alleviate anxiety-like behaviors in PTSD-like mice,and its mechanism of action may be closely related to the inhibition of inflammatory immune response.
