Morphological study of afferent connections of VGLUT2 positive neurons of mediodorsal thalamic nucleus in mice
10.16557/j.cnki.1000-7547.2024.03.002
- VernacularTitle:小鼠丘脑背内侧核内VGLUT2阳性神经元传入联系的形态学研究
- Author:
Huijie FENG
1
,
2
;
Fei PENG
;
Meiqi XUE
;
Cailian RUAN
;
Yulin DONG
Author Information
1. 空军军医大学基础医学院人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安 710032
2. 延安大学基础医学院人体解剖学教研室,延安 716000
- Keywords:
mediodorsal thalamic nucleus(MD);
rabies virus(RV);
vesicular glutamate transporters 2(VGLUT2);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(3):287-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Using retrogradely trans-monosynaptic tracing method mediated by rabies virus(RV)to ob-serve presynaptic neuronal distributions of vesicular glutamate transporter 2(VGLUT2)-positive neurons of the med-iodorsal thalamic nucleus(MD)in whole brain.Methods:The helper viruses of RV were injected into the right MD of VGLUT2-ires-Cre mice,and two weeks later RV was injected into the same area.After another one week,perfusion was taken and whole brain scanning was performed to observe the distributions of RV-labeled presynaptic neurons throughout the brain.Results:After RV virus was injected into the MD of VGLUT2-ires-Cre mice,dense presynaptic neurons were observed in the cortex and brainstem.RV labeled neurons were mostly distributed in primary motor cortex(M1),sec-ondary motor cortex(M2),medial prefrontal cortex,orbitofrontal cortex and insular cortex at the cortical level.Tha-lamic retrogradely labeled neurons were mostly found in reticular thalamic nucleus and lateral hypothalamic area,and retrogradely labeled neurons in the brainstem were mainly distributed in lateral parabrachial nucleus,periaqueductal grey matter and dorsal raphe nucleus.Conclusion:The results in the present experiment suggest that VGLUT2-positive neurons within the MD can receive ascending information transmission from the brainstem,or regulation from the reticu-lar nucleus of thalamus.As a higher-order thalamus,MD can also receive descending projections from the cortex,and is involved in a variety of functions.Our results provide morphological basis for the study of the function and the related neural circuit of the MD.
