Chronic intermittent hypoxia impairs learning and memory by upregulating HMGB1 and NF-κB in rat hippocampus
10.16557/j.cnki.1000-7547.2024.02.012
- VernacularTitle:慢性间断缺氧通过上调大鼠海马HMGB1和NF-κB损伤学习记忆
- Author:
Zhengang WU
1
;
Yao XIAO
;
Yafang CHEN
;
Jinying ZHANG
;
Zeming GUO
;
Jun LIN
;
Meili YANG
Author Information
1. 泉州医学高等专科学校外科教研室,泉州 362000
- Keywords:
chronic intermittent hypoxia(CIH);
high mobility group box-1(HMGB1);
nuclear transcription fac-tor-κB(NF-κB);
learning and memory;
hippocampus;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(2):224-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of chronic intermittent hypoxia(CIH)on learning and memory dysfunc-tion in rats,as well as the expression of high mobility group box-1(HMGB1)and nuclear transcription factor-KB(NF-κB)in the hippocampus region.Methods:The CIH rat model was established,and forty SD rats were randomly divid-ed into four groups:normoxia group,hypoxia for 4 weeks group(CIH4 group),hypoxia for 8 weeks group(CIH8 group),and hypoxia for 12 weeks group(CIH12 group).Morris water maze was used to assess the learning memory ability of rats,and immunohistochemistry and ELISA were used to detect the expression of HMGB1 and NF-κB in the hippocampus of rats.Results:Compared with the normoxia group,the CIH12 and CIH8 groups had longer escape la-tency,the number of crossing the platform and the residence time in the quadrant of the platform were significantly shortened,but there was no significant difference in the CIH4 group.Additionally,there was no significant expression of HMGB1 and NF-κB in the hippocampal region of the normoxia group,but little expression was observed in CIH4 group,and significantly expressed in CIH8 group and CIH12 group,and the expression of CIH12 group was significantly higher than that of CIH8 group.Conclusion:CIH can lead to a decline in learning and memory function in rats,and the longer time of intermittent hypoxia led to the more significant effect on their learning and memory function.In addi-tion,CIH also leads to increased expression levels of HMGB1 and NF-κB in the hippocampus region,and the expres-sion increased more significantly after hypoxia for 12 weeks,comparing to hypoxia for 8 weeks.
