Brilliant blue G alleviates neuroinflammation in cerebral ischemia-reperfusion rats by inhibiting the P2X7/NLRP3 pathway
10.16557/j.cnki.1000-7547.2024.02.011
- VernacularTitle:亮蓝G通过抑制P2X7/NLRP3通路减轻脑缺血再灌注大鼠神经炎症
- Author:
Zhen WANG
1
;
Chong ZHANG
;
Zuolin SHI
;
Yushu DONG
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Keywords:
cerebral ischemia-reperfusion injury(CIRI);
P2X7/NLRP3 signal pathway;
brilliant blue G(BBG);
microglia;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(2):219-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of purine receptor P2X7 in cerebral ischemia-reperfusion injury(CI-RI).Methods:The CIRI model was prepared by right middle cerebral artery occlusion(MCAO).The P2X7 inhibitor bright blue G(BBG)or NLRP3 inhibitor MCC950 were injected intraperitoneally.Neurological function score was used to detect the changes of neural function in rats.The integrity of blood-brain barrier was detected by Evans blue staining.The contents of IL-1 β and IL-18 in cerebrospinal fluid(CSF)of rats were detected by ELISA,and the expressions of P2X7,CD11b,and NLRP3 in the right cerebral cortex were detected by Western Blot.Results:Treatment with BBG or MCC950 improved neural function in CIRI rats,while reducing the amount of Evans blue in brain tissue and IL-1 β and IL-18 levels in CSF.In addition,BBG can reduce the expression of CD11b and NLRP3 in the right cerebral cortex,while MCC950 can only reduce the expression of CD11b,but has no effect on P2X7 expression.Conclusion:BBG alle-viates neuroinflammation in CIRI rats by inhibiting P2X7/NLRP3 pathway.
