Panax notoginseng saponins inhibit LPS-induced microglia activation through p38 MAPK pathway
10.16557/j.cnki.1000-7547.2024.02.008
- VernacularTitle:三七总皂苷通过p38 MAPK通路抑制内毒素诱导的小胶质细胞活化
- Author:
Zhaoda DUAN
1
;
Jianxiang WANG
;
Li YANG
;
Dongyao XU
;
Zhi QI
;
Chunyun WU
;
Wenji JIA
Author Information
1. 昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明 650500
- Keywords:
panax notoginseng saponins(PNS);
p38 mitogen-activated protein kinase(p38 MAPK);
lipopolysac-charide(LPS);
tumor necrosis factor-α(TNF-α);
BV2 microglia
- From:
Chinese Journal of Neuroanatomy
2024;40(2):196-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of panax notoginseng saponins(PNS)on the expression of tumor necrosis factor-α(TNF-α)in lipopolysaccharide(LPS)-induced activated BV2 microglia through p38 mitogen-activa-ted protein kinase(p38 MAPK)pathway.Methods:BV2 microglia were divided into control group,LPS activated group and LPS+panax notoginseng saponins intervention group(LPS+PNS).The CCK-8 method was used to detect the viability of BV2 microglia and determine the optimal drug intervention concentration.Western Blot and immunofluo-rescence were used to detect the expression of p38 MAPK and TNF-α and the phosphorylation level of p38 MAPK(p-p38 MAPK)in BV2 microglia.Results:Compared with the blank control group,there was no significant difference in the cell viability of BV2 microglia,and finally 100 mg/L was selected as the drug intervention concentration.Western Blot and immunofluorescence results indicated that after LPS activation,the expression of TNF-α and the phosphoryla-tion level of p38 MAPK in BV2 microglia were significantly increased(P＜0.05).After PNS intervention,compared with LPS-activated group,the expression of TNF-α and the phosphorylation level of p38 MAPK were significantly decreased(P＜0.05).After treatment with p38 MAPK pathway inhibitor(SB203580),there was no significant differ-ence in the expression levels of p-p38 MAPK and TNF-α in PNS combined with SB203580 group(LPS+PNS+I)com-pared with LPS+PNS group(P＞0.05).In addition,the changes of p38 MAPK in each group were not statistically sig-nificant(P＞0.05).Conclusion:PNS may inhibit the expression of inflammatory factor TNF-α secreted by activated BV2 microglia through p38 MAPK pathway.
