Medial prefrontal cortex neurons are involved in diphenylcycloacrylonone induced itch in mice
10.16557/j.cnki.1000-7547.2024.02.001
- VernacularTitle:内侧前额叶皮质神经元参与二苯基环丙烯酮诱导的小鼠瘙痒
- Author:
Jingwen LIU
1
,
2
;
Yuanyuan ZHU
;
Ziyi DAI
;
Jing HUANG
;
Zhantao BAI
Author Information
1. 延安大学生命科学学院多肽资源药物研究中心,陕西省区域生物资源保育与利用工程技术研究中心,延安 716000
2. 空军军医大学基础医学院神经生物学教研室,西安 710032
- Keywords:
contact dermatitis;
pruritus;
medial prefrontal cortex(mPFC);
c-Fos;
p-ERK1/2;
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(2):137-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish contact dermatitis pruritus model and detect itch,anxiety and depression-like behaviors.To observe the activation of the medial prefrontal cortex(mPFC)neurons and provide morphological evi-dence for its involvement in the regulation of itch perception information.Methods:A model of contact dermatitis pruri-tus was constructed with diphenylcyclopyrenone(DCP)applied on the neck and back of the mouse,and the itch per-ception video tracking system was used to detect the scratching behavior.Open field and suspended tail behavioral ex-periments were conducted to explore the anxiety and depression-like behaviors of mice under contact dermatitis.The dis-tribution of c-Fos and p-ERK1/2 positive cells in the mPFC of contact dermatitis model mice was investigated by immu-nofluorescence staining.Results:The contact dermatitis pruritus model was successfully established,and the bouts of scratching in the contact dermatitis model group was significantly higher than that in the control group(P＜0.05).The results of open field test showed that the time and distances were significantly reduced in the central area of the contact dermatitis model group.The results of the tail suspension experiment suggested that the time of immobility increased sig-nificantly in the contact dermatitis model group(P＜0.05).There were more c-Fos positive and p-ERK1/2 positive cells in the mPFC compared with the control group(P＜0.05).Conclusion:Bouts of scratching were increased in the contact dermatitis group and the mice had anxiety and depression-like behaviors.In the contact dermatitis state,mPFC neurons were activated,providing morphological evidence for the involvement of mPFC neurons in contact dermatitis pruritus and accompanying affective disorders such as anxiety and depression.
