The construction and identification of adult-derived placental site trophoblastic tumor organoid
10.3969/j.issn.1672-8467.2024.05.023
- VernacularTitle:人源胎盘部位滋养细胞肿瘤类器官模型的构建与鉴定
- Author:
Sai ZHANG
1
;
Jia-Yi ZHOU
;
Jing WU
;
Huan-Di YU
;
Yu-Xiao DING
;
Yan DU
;
Xin LU
;
Hong-Bo ZHAO
Author Information
1. 复旦大学附属妇产科医院生物样本库 上海 200011
- Keywords:
organoid;
placental site trophoblastic tumor(PSTT);
cell culture
- From:
Fudan University Journal of Medical Sciences
2024;51(5):800-806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify an organoid model of human placental site trophoblastic tumor(PSTT).Methods The tumor cells were obtained by digesting and separating the PSTT tissues and then embedded in Matrigel.The organoids were cultured in the specific organoid medium.The histological morphology of the organoid model was observed by HE staining and the expression levels of the PSTT specific markers[human placental prolactin(HPL),human leukocyte antigen-G(HLA-G)and placental alkaline phosphatase(PLAP)]were detected by immunohistochemistry and immunofluorescence,so as to evaluate the consistency between the organoid model and the PSTT tissue.Meanwhile,the morphology and forming efficiency of the constructed model were observed under a microscope after primary culture,passage generation and cryopreservation to evaluate its potential application as an organoid model in basic and clinical translational research of PSTT.Results The constructed organoid model could proliferate stably,growing from small microspheres into compact solid spheres or spheres with follicle-like structures,and could passage after fully grown in 7-10 days.The cell state remained stable after passage,frozen storage and recovery.HE staining showed that the morphology of the cells in the organoids was similar to that of the primary PSTT tumor cells,and immunofluorescence staining showed that the organoids highly expressed HLA-G and lowly expressed β-HCG,indicating that the constructed organoid model mainly contained intermediate trophoblast.Conclusion The adult-derived PSTT organoid(ADPO)models were successfully established.
