Protective effects of mild hypothermia and/or felbamate on hypoxic-ischemic brain damage in newborn rats
10.3760/j.issn:0578-1310.2001.01.004
- VernacularTitle:亚低温和非氨酯对新生鼠缺氧缺血性脑损伤的保护作用
- Author:
Mei QIN
1
;
Shaozeng FAN
Author Information
1. Children's Hospital Medical Center of Fudan University
- From:
Chinese Journal of Pediatrics
2001;39(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of mild hypothermia and/or felbamate on hypoxic-ischemic (HI) brain damage. Methods The HI model rats (subjected to unilateral right carotid artery ligation and given 8% oxygen for 2 h) were allocated to mild hypothermia groups （the brain temperature dropped by 4.5℃) for 24 h course (Hy24，n=26) and 72 h course (Hy72, n=48) , felbamate group (Fbm, n=48), Hy24 combined with Fbm group (HF, n=51) and placebo group (Pla, n=48). The control group (Con, n=42) was shame-operated animals. The rectal temperature (RT) and brain temperature (BT) of experimental animals were monitored. The plasma S-100 and CK-BB levels, brain injury scores (BIS) and percentage of hippocampal dead neurons (HDNP) and the behavioral test of Morris Water Maze were used to assess the protective effects. Results BT was 0.3～0.6℃ which was lower than RT (r=0.99, P＜0.01). RT dropped by 4～5℃in Hy24, Hy72 and HF groups. RT maintained in 36～37℃in Fbm and Pla groups. The peak levels of plasma S-100 and CK-BB in Pla group (1.21～1.24 μg/L and 52.9～54.2 IU/L, respectively) were about 2 to 3 times as high as normal levels between 24 h to 48 h after HI, while the plasma S-100 and CK-BB levels in Hy24，Hy72, Fbm and HF groups were lower than Pla group (P＜0.01). At 72 h, 14d and 60d after HI, the reductions, of BIS and HDNP in Hy72 group were 58%, 85% and 87%, HF group 56%, 77% and 76%, Hy24 group 35%, 58% and 47% and Fbm group 35%, 66% and 67%, respectively. The order of spatial remember function was Con, and HF>Hy72>Hy24≥Fbm>Pla. Conclusions Mild hypothermia and/or felbamate showed different protective effects on brain HI damage as reflected by the changes of the plasma S-100 and CK-BB levels, the results of behavior assessment and histopathological changes. The optimal effect was obtained when hypothermia and felbamate were used simultaneously.
