Contribution of microglia in the basolateral amygdala to pain hypersensitivity and pain-related aversion in mouse model of monoarthritis
10.3969/j.issn.1672-8467.2024.03.001
- VernacularTitle:基底外侧杏仁核小胶质细胞参与单关节炎小鼠痛觉敏化和痛相关厌恶行为
- Author:
Hong LIN
1
;
Tian-Le SHI
;
Yu-Qiu ZHANG
;
Hong CAO
Author Information
1. 复旦大学脑科学研究院 上海 200032
- Keywords:
basolateral amygdala(BLA);
microglia;
hyperalgesia;
pain-related aversion;
arthritis;
mouse
- From:
Fudan University Journal of Medical Sciences
2024;51(3):285-294
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the contribution of microglia in the basolateral amygdala(BLA)to pain hypersensitivity and pain-related aversion in knee-joint monoarthritis mice.Methods A total of 61 mice were used for behavioral tests(14 mice in the control group and 47 mice in the model group),and other 6 mice were used for cell morphology(3 mice in each group).An animal model of knee-joint monoarthritis was established by injection of complete Freund's adjuvant(CFA)into the knee-joint cavity of mice.The von Frey and Hargreaves tests were used to examine mechanical allodynia and thermal hyperalgesia in mice,respectively.The place escape/avoidance paradigm test was used to examine pain-related aversion.Open field test and elevated plus maze test were used to examine anxiety-like behaviors in mice.Morphological changes of microglia in the BLA area after CFA injection were assessed by 3D reconstruction of microglia in the BLA brain region using immunofluorescence staining and Imaris software.Results Compared with the control group,CFA-arthritic mice produced significant mechanical and thermal hyperalgesia in the ipsilateral hindpaw and maintained for at least 12 and 19 days,respectively.Meanwhile,CFA injection induced pain-related aversion and anxiety-like behaviors in mice,accompanied by significant activation of BLA microglia.Inhibition of BLA microglia activation alleviated CFA-induced hyperalgesia and aversive behaviors but had no significant effects on anxiety-like behaviors.Conclusion CFA-arthritic mice produce hyperalgesia,pain-related aversion,and anxious behavior,in which hyperalgesia and pain-related aversion may be mediated by the activation of microglia in BLA.
