Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia
10.3321/j.issn:0371-0874.2001.02.008
- VernacularTitle:缺血引起沙土鼠纹状体DARPP-32磷酸化状态的变化
- Author:
Ya-Feng SUN
1
;
Fang-Ming TANG
;
Ran WANG
;
Guang-Yi ZHANG
;
Guo-Zhang JIN
Author Information
1. Xuzhou Medical College
- From:
Acta Physiologica Sinica
2001;53(2):108-110
- CountryChina
- Language:Chinese
-
Abstract:
Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP-32 in striatum were measured by back-phosphorylation and Western-blotting assay in vitro. Transient forebrain ischemia had no effect on the immunoreaction of DARPP-32 in striatum, but the state of phosphorylation of DARPP-32 showed a significant change. The ［32P］ phosphate incorporation in DARPP-32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5-min ischemia. In contrast, the result of back-phosphorylation in vivo was opposite to that obtained in vitro.
