Substance P depresses GABA-activated currents in cultured hippocampal pyramidal neurons of rats
10.3321/j.issn:0371-0874.2001.02.007
- VernacularTitle:P物质抑制培养大鼠海马大锥体细胞GABA-激活电流
- Author:
Shun-Hua XIONG
1
;
ZHI-WANG LI
;
You-Zhen FAN
;
Ming-Jiang WANG
;
Jin-Bo WEI
Author Information
1. Yunyang Medical College
- From:
Acta Physiologica Sinica
2001;53(2):103-107
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the present study was to explore whether substance P (SP) modulates the response mediated by GABAA receptors. Experiments were carried out on cultured hippocampal pyramidal neurons of rats. GABA-activated inward currents were recorded using the whole-cell-patch-clamp techique. The majority of the neurons examined (66/92, 72%) were sensitive to both GABA and SP. When the neurons were treated with SP prior to application of GABA, the GABA-activated current (IGABA) was inhibited markedly, which was concentration-dependent and could be blocked by spantide, an NK1 receptor antagonist. With 10－8, 10－7, 10－6 and 10－5 mol/L SP, IGABA was inhibited by 18%, 24.8%, 25.9% and 28% respectively. Intracellular application of H7, a potent inhibitor of PKC, abolished inhibition of IGABA by SP, suggesting that the inhibition of IGABA by SP may be a result of intracellular phosphorylation of the GABAA receptor.
