Inhibitory effect of genistein on the proliferation of the anterior pituitary cells of rats
10.3321/j.issn:0371-0874.2001.01.013
- VernacularTitle:Genistein对大鼠垂体前叶细胞增殖的抑制作用
- Author:
Qing-Hong ZHANG
1
;
Yu-Zhen HU
;
Shi-Sheng ZHOU
;
Fu-Zhou WANG
Author Information
1. Fourth Military Medical University
- From:
Acta Physiologica Sinica
2001;53(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
The effects of tyrosine kinase inhibitor genistein on the proliferation of rat anterior pituitary cells and mouse AtT-20 cells were studied using techniques of cell culture, ～3H-TdR incorporation, flow cytometric analysis and electron microscope. Genistein significantly inhibited the proliferation of rat anterior pituitary cells and mouse AtT-20 cells. Genistein (50 and 100 μmol/L ) blocked the proliferation of AtT-20 cells at G0/G1 and G2/M phases and evoked an apoptotic peak of these cells with an apoptotic ratio of 19.9％ and 36.4%. The apoptotic cells were also observed under the electron microscope. In consequence, genistein, as a tyrosine kinase inhibitor, can significantly inhibit the proliferation of pituitary cells possibly by inducing apoptosis, and the tyrosine kinase activity may play a key role in the proliferation and differentiation of pituitary cells.
