Effect of aldosterone on the secretion of endothelin by ventricular fibroblasts
10.3321/j.issn:0371-0874.2001.01.007
- VernacularTitle:醛固酮对心室成纤维细胞分泌内皮素的影响
- Author:
Su-Zhen GONG
1
;
Pei-Qing LIU
;
Wei LU
;
Ting-Huai WANG
;
Shi-Gan FU
;
Zhi TAN
;
Jing-Yun PAN
Author Information
1. Sun Yat-Sen University of Medical Sciences
- From:
Acta Physiologica Sinica
2001;53(1):23-26
- CountryChina
- Language:Chinese
-
Abstract:
Using cell culture, radioimmunoassay for endothelin and RT-PCR, the effect of aldosterone on the endothelin secretion of ventricular fibroblasts was studied. The results showed that aldosterone (1×10－7 mol/L) promoted the expression of ppET-1 mRNA, which began to increase in 2 hours and attained the highest level in 4 hours, thereafter decreased; aldosterone increased the endothelin level in ventricular fibroblasts and fibroblast conditioned growth medium (FCGM) as well, which was blocked by spironolactone (1×10－6 mol/L), an aldosterone receptor antagonist. The results suggest that aldosterone can increase endothelin secretion by ventricular fibroblasts, which can be inhibited by its receptor antagonist spironolactone.
