GABAergic neurons in K(o)lliker-Fuse nucleus and B(o)tzinger complex with axons projecting to phrenic nucleus
10.3321/j.issn:0371-0874.2000.02.017
- VernacularTitle:KF核及B(o)tzinger复合体内GABA能神经元向膈神经核的投射
- Author:
Gang SONG
1
;
Qin LI
;
Feng-Zhi SHAO
Author Information
1. 山东医科大学
- Keywords:
GABAergic neuron;
K(o)lliker-Fuse nucleus;
B(o)tzinger complex;
phrenic nucleus;
anti-GABA antibody;
cat
- From:
Acta Physiologica Sinica
2000;52(2):167-169
- CountryChina
- Language:Chinese
-
Abstract:
Experiments were made on 6 adult cats. Brainstem neurons that project to the phrenic nucleus were retrogradely labeled by microinjection of WGA-HRP into phrenic nucleus. The brainstem sections were doubly processed for HRP histochemical staining with the TMB-Co-DAB method, and GABA immunohistochemical staining with anti-GABA primary antibody and FITC conjugated immunofluorescent secondary antibody. HRP-FITC double-labeled neurons were observed in the pontine K(o)lliker-Fuse nucleus and areas around the retrofacial nucleus (B(o)tzinger complex). Double-labeled neurons were also observed in the raphe nucleus, the lateral paragigantocellular nucleus, and the vestibular nucleus. The results show that brainstem GABAergic neurons in these structures, especially those in the K(o)lliker-Fuse nucleus and the B(o)t.C, send axonal projections to the phrenic nucleus.
