CHARACTERISTICS OF APYRASE (EC 3.6.1.5) ON CULTURED BOVINE ENDOCARDIAL ENDOTHELIAL CELLS

VernacularTitle:培养牛心内膜内皮细胞的腺苷三磷酸双磷酸酶的特性研究
Author: FU-XIAN YI ¹ ; PING SUN ; SHAO-LING HUANG ; WEN-LAN LIU ; ZHAO-GUI GUO
Author Information

1. Hunan Medical University
Keywords: apyrase; adenosine diphosphate; endocardium; sodium azide
From: Acta Physiologica Sinica 1999;51(4):425-429
CountryChina
Language:Chinese
Abstract: Apyrase activities in some tissues and cells, such as peripheral vascular endothelial cells, have been reported, but these in endocardium endothelial cells have not been reported. The present study was to characterise the properties of bovine endocardium endothelial cells (BEEC)-associated apyrase. Apyrase activity was assayed by inorganic phosphate release, which could be inhibited concentration-dependently by NaN3, an apyrase inhibitor. NaF (20 mmol/L), another inhibitor of apyrase, also markedly inhibited the activity. EDTA or EGTA (1 mmol/L) could also inhibit the activity completely. However, the inhibitor for Na+/K+-ATPase, ouabain (3 mmol/L) did not affect the enzyme activity. BEEC apyrase activity was dependent on divalent cations (Ca2+ or Mg2+) and pH value.