MODULATION OF GLYCINE-ACTIVATED CHLORIDE CURRENTS BY SUBSTANCE P IN RAT SACRAL DORSAL COMMISSURAL NEURONS
10.3321/j.issn:0371-0874.1999.04.001
- VernacularTitle:P物质对大鼠骶髓后连合核神经元甘氨酸激活的氯电流的调控
- Author:
DIAN-SHI WANG
1
;
TIAN-LE XU
;
JI-SHUO LI
Author Information
1. Fourth Military Medical University
- Keywords:
substance P;
glycine;
sacral dorsal commissural nucleus;
protein kinase C;
Ca2+/calmodulin-dependent protein kinase Ⅱ
- From:
Acta Physiologica Sinica
1999;51(4):361-370
- CountryChina
- Language:Chinese
-
Abstract:
The modulatory effect of substance P (SP) on strychnine-sensitive glycine (Gly) response was examined in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using nystatin perforated patch recording configuration under voltage-clamp conditions. Application of SP potentiated 30 μmol/L Gly-activated chloride current (IGly) in a concentration-dependent manner over the range of 1 nmol/L to 1 μmol/L at a holding potential of -40 mV. SP neither changed the reversal potential of Gly response nor affected the affinity of Gly to its receptor. The SP potentiation effect could be blocked by spantide as well as a selective NK1 receptor antagonist, L-668,169, but not by NK2 receptor antagonist, L-659,877. The facilitatory action of SP on IGly could also be abolished by pretreatment with chelerythrine or KN-62 in different neurons, a finding suggesting that protein kinase C (PKC) or Ca2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) possibly contributes to an intracellular pathway of SP in the augmentation of IGly. The results imply that SP may suppress nociception in the spinal cord by potentiating Gly response.
