ANTIPYRETIC ACTION OF DEXAMETHASONE ON EGTAZIC ACID-INDUCED FEVER IN RABBITS
10.3321/j.issn:0371-0874.1999.02.007
- VernacularTitle:地塞米松对家兔乙二醇双(2-氨基乙醚)四乙酸性发热的解热作用
- Author:
HUA-DONG WANG
1
;
YAN-PING WANG
;
CHU-JIE LI
;
DA-XIANG LU
;
LIANG YAN
;
REN-BIN QI
Author Information
1. Medical College of Jinan University
- Keywords:
fever;
egtazic acid;
glucocorticoid;
actinomycin D;
calcium;
dexamethasone
- From:
Acta Physiologica Sinica
1999;(2):161-167
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the present study was to investigate whether the antipyretic effect of dexamethasone (DEX) delivered by intravenous injection (iv) on intracerebroventricularly (icv) administered egtazic acid-induced febrile response is relevant to the changes in cytosolic free calcium concentration of the hypothalamus. The colon temperatures were measured by a thermistor and the cytosolic free calcium concentration ([Ca2+]i) in dissociated brain cells was measured by Fura 2-AM. The results demonstrated that the pyretic action of egtazic acid (0.6 μmol, icv) was markedly inhibited by DEX (5 mg/kg,iv), but DEX (60～120 μmol/L) did′t affect [Ca2+]i in dissociated hypothalamus cells. Actinomycin D, which interferes with gene transcription (3 nmol, icv), completely abolished the antipyretic action of DEX on egtazic acid-induced fever. These findings suggest that the antipyretic action of DEX on egtazic acid-induced fever is related to the activation of certain gene expression in the brain,but not to the changes of transmembrane calcium ion current in hypothalamus neurons.
