Zn2+ DEPRESSES GABAA RECEPTOR MEDIATED RESPONSES IN ACUTELY DISSOCIATED SACRAL DORSAL COMMISSURAL NEURONS

VernacularTitle:锌离子对急性分离的大鼠骶髓后连合核神经元GABAA受体介导电流的抑制作用
Author: ZHI-PING PANG ¹ ; DIAN-SHI WANG ; TIAN-LE XU ; Ji-Shuo LI
Author Information

1. 第四军医大学
Keywords: zinc; GABAA-receptor; nystatin-perforated whole cell recording; dissociated cells; sacral dorsal commissural nucleus
From: Acta Physiologica Sinica 1998;50(6):649-655
CountryChina
Language:Chinese
Abstract: The effects of Zn2 + on GABAA-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques.The results demonstrated that ( 1 ) GABA induced inward currents through activation of GABAA-receptor at a holding potential of -40 mV; (2) GABAA-receptor mediated responses were suppressed by Zn2 + in a reversible and voltage-independent manner; and (3) in the presence of Zn2 + , the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn2 +allosterically depresses GABAA-receptor mediated currents.