THE CHANGES OF AP-1 DNA BINDING ACTIVITY AND COMPONENTS IN HIPPOCAMPUS OF SEIZURE-SENSITIVE RAT INDUCED BY KAINATE
10.3321/j.issn:0371-0874.1998.04.005
- VernacularTitle:红藻氨酸致敏癫痫大鼠海马内AP-1 DNA结合活性和成分的改变
- Author:
Dong-Dong LI
1
;
Ze-Hui FENG
;
Wan-Qin ZHANG
;
JAU-SHYONG HONG
Author Information
1. 大连医科大学
- Keywords:
AP-1 binding activity;
AP-1 complex;
seizure susceptibility;
hippocampus
- From:
Acta Physiologica Sinica
1998;50(4):385-391
- CountryChina
- Language:Chinese
-
Abstract:
Seizure-sensitive Fisher 344 rats were prepared 7 d after seizure episodes induced by a single subcutaneous administration of convulsive dose (7.5 mg/kg)of kainic acid. The activator protein-1 (AP-1) DNA binding activity and its components in the hippocampus of such rats were examined by Gel shift,Super-shift and Western blot assay. Gel shift assay showed that the basal level of AP-1 DNA binding activity was significantly higher than that of control rat hippocampus. Super-shift showed that only Fra and Jun D antibodies could super-shift AP-1 complex to a higher position. It suggested that the AP-1 complex was composed of Fra and Jun D in hippocampus of the seizure-sensitive rats. Western blot analysis further confirmed that Jun D proteins contained components of 43,39 and 28 kDa. When secondary seizure stimulations were given to seizure-sensitive rats, the AP-1 DNA binding activity was attenuated and the components of AP-1proteins changed transiently. In light of our results and those of literature, it appears that all the mentioned changes may play an important role in induction of long-lasting enhancement in seizure susceptibility.
