Association of HIF- expression and cell apoptosis after traumatic brain injury in the rat
- Author:
Rutong YU
1
;
Lida GAO
;
Shu JIANG
;
Peng GUAN
;
Boyong MAO
Author Information
1. Affiliated Hospital of Xuzhou Medical College
- Keywords:
Brain injuries;
Apoptosis;
Hypoxiainducible factor-1α
- From:
Chinese Journal of Traumatology
2001;4(4):218-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the expression of hypoxia inducible factor-1α (HIF-1~) and the correlation between HIF-1α and apoptosis after traumatic brain injury.Methods: Using experimental traumatic brain injury in the rats, the expression of HIF-1α was studied by immunohisto-chemistry in cerebral tissue, apoptotic cell death was evaluated with TUNEL (transferase-mediated XdUTP nick end labeling ), and double-labeled immunohistochemistry and TUNEL methods were used to investigate the relationship between HIF-1α and apoptosis.Results: There was remarkable difference in the expression of HIF-1α between the experimental groups and the control groups (P ＜ 0.01), in the experimental groups,the expression of HIF-1α at 48 hours was highest; the evidence of apoptotic cell death after experimental traumatic brain injury was found by TUNEL; the apoptotic percentage increased or decreased according to the changes of the positive expression of HIF-1α (r = 0.99).Conclusions: The results suggest that secondary brain ischemia plays a crucial role in apoptotic cell death after traumatic brain injury; HIF-1α can prompt apoptotic cell death after experimental traumatic brain injury.
