Signal transduction pathway of nitric oxide inducing PC12 cell death
- Author:
Chengyi LUO
1
;
Ruxiang XU
;
Zhilin YANG
Author Information
1. 第一军医大学附属珠江医院
- Keywords:
Transduction,signal;
PC12 cells;
Cell death;
Nitric oxide
- From:
Chinese Journal of Traumatology
2001;4(2):86-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study signal transduction pathway of nitric oxideinducing death of PC12 cells.Methods: Cell survival rate was measured with MTT assay, and caspase-3 activity with caspase-3 assay kits after PC12 cells were incubated with sodium nitroprusside (SNP), caspase-3 inhibitor Ⅱ plus SNP or p38 inhibitor-SB203580 plus SNP.Results: SNP induced death of PC12 cells in dose- and time-dependent manner and enhanced caspase-3 activity gradually. Both caspase-3 inhibitor Ⅱ and SB203580 reduced cell death, but SB203580 reduced caspase-3 activity significantly.Conclusions: NO may induce death of PC12 cells through activation of p38 and caspase-3.
