Expression of TFF3 in gastric cancer and its effect on proliferation of gastric cancer cells
10.3760/cma.j.cn115807-20240109-00009-1
- VernacularTitle:TFF3在胃癌中的表达及对胃癌细胞增殖的影响
- Author:
Libin ZHANG
1
;
Xing CHEN
;
Quanmao ZHANG
;
Yong ZHANG
Author Information
1. 山西省肿瘤医院 中国医学科学院肿瘤医院山西医院 山西医科大学附属肿瘤医院内镜中心，太原 030013
- Keywords:
Gastric cancer;
Trefoil factor 3;
Vascular endothelial growth factor
- From:
Chinese Journal of Endocrine Surgery
2024;18(5):744-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of trefoil factor 3 (TFF3) in gastric cancer and its regulation mechanism on the gastric cancer cells proliferation.Methods:The differences in the expression levels of TFF3 in 66 cases of gastric cancer were detected by RT-PCR and immunohistochemical method. SGC-7901 cells were cultured stably. Through the expression of TFF3 gene in silent cells infected with gv248 lentivirus vector (SGC-7901/KD group) and the setting up of control group (SGC-7901/NC group), CCK-8 method, flow cytometry and Transwell method were used to detect the changes of cell activity. SGC-7901/KD or SGC-7901/NC cells were injected into the right armpit of 20 BALB/C nude mice respectively. The expression of VEGF in tumor was detected by immunohistochemical method and Western blot method.Results:The expression level of TFF3 mRNA in gastric cancer tissues (15.31±2.18) was significantly higher than that in adjacent tissues (4.58±1.69) ( P < 0.05). After infection with recombinant lectin virus, the expression level of TFF3 gene in SGC-7901/KD cells was 0.08±0.006, significantly lower than that in the other two groups. After 5 days of culture, the OD value of cells in SGC-7901/KD group was 1.28±0.05, significantly lower than that in SGC-7901/NC group (1.78±0.08). The number of cell colonies formed was also significantly reduced, and the colonies were smaller. Compared with the control group, the proportion and apoptosis rate of SGC-7901/KD cells in G0/G1 phase were significantly higher, while the proportion of cells in G2/M phase was significantly lower. The results of scratch healing test showed that the distance between wound edges of SGC-7901/KD cells was significantly shorter than that of SGC-7901/NC cells. Western blot analysis showed that the relative expression level of VEGF protein in SGC-7901/KD cells (0.98±0.05) was significantly lower than that in SGC-7901/NC cells (2.87±0.17) ( P < 0.05). Compared with SGC7901/NC group, after 2 weeks of injection of SGC-7901/KD cells, the tumor volume of mice was (184.4±50.2) mm 3, significantly smaller than that of SGC7901/NC group (326.8±76.9) mm 3 ( P < 0.05). The expression of VEGF protein in SGC7901/KD group was significantly lower than that in control group ( P < 0.05) . Conclusion:In gastric cancer cells, TFF3 gene silencing can inhibit the growth of gastric cancer cells by inhibiting the expression of VEGF, which is expected to become a new molecular target.
