Expression and diagnostic value of DDIT3 immunohistochemical antibody in 53 cases of myxoid liposarcoma
10.3760/cma.j.cn112151-20221030-00899
- VernacularTitle:DDIT3免疫组织化学抗体在53例黏液样脂肪肉瘤中的表达及诊断价值
- Author:
Mengmeng TIAN
1
;
Ming ZHANG
;
Xiaoqi SUN
;
Lan LI
;
Yi DING
Author Information
1. 北京积水潭医院 北京大学第四临床医学院病理科，北京 100035
- Keywords:
Soft tissue neoplasms;
Immunohistochemistry;
In situ hybridization, fluorescence;
Myxoid liposarcoma
- From:
Chinese Journal of Pathology
2023;52(3):280-284
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the diagnostic value of DDIT3 immunohistochemical antibody in myxoid liposarcoma.Methods:A total of 53 patients diagnosed with myxoid liposarcoma and with DDIT3 gene rearrangement detected by fluorescence in situ hybridization (FISH) from 2010 to 2022 in Beijing Jishuitan Hospital were enrolled as the case group, and 50 patients with other tumors that needed to be differentiated from myxoid liposarcoma during the same period were enrolled as the control group. The immunochemical (IHC) expression of DDIT3 was evaluated in all cases and the specificity and sensitivity in the diagnosis of myxoid liposarcoma were calculated. The correlation between IHC DDIT3 expression and FISH detection of DDIT3 gene rearrangement was analyzed.Results:Among the 53 cases of myxoid liposarcoma, thirty-one patients were males and twenty-two were females. The patients ranged in age from 11 to 74 years, with a median age of 51 and an average age of 49.4 years. The expression rate of IHC DDIT3 expression in 53 cases of myxoid liposarcoma was 100%. No expression was found or only scattered individual cell (<10%) expression was seen in a very few cases in the. control group. There was no significant correlation between the proportion and intensity of IHC and FISH DDIT3 positive cells or the partner gene.Conclusions:The sensitivity and specificity of DDIT3 immunohistochemical antibody in the diagnosis of myxoid liposarcoma are both 100%, which are consistent with the results of FISH. It can be used as a convenient and economical diagnostic tool for myxoid liposarcoma.
