Application of immunohistochemical staining of PRAME in differential diagnosis between melanoma and melanocytic nevus
10.3760/cma.j.cn112151-20211116-00835
- VernacularTitle:黑色素瘤优先表达抗原免疫组织化学染色在良恶性皮肤黑色素细胞肿瘤鉴别中的应用
- Author:
Juan DU
1
;
Wenjuan YU
;
Ruiping GUO
;
Jing SU
Author Information
1. 北京大学第三医院病理科 北京大学基础医学院病理学系，北京100191
- Keywords:
Melanoma;
Immunohistochemistry;
Diagnosis, differential;
Melanocytic nevus;
PRAME
- From:
Chinese Journal of Pathology
2022;51(7):621-626
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the diagnostic value of preferentially expressed antigen in melanoma (PRAME) in differential diagnosis of benign and malignant cutaneous melanocytic lesions.Methods:Fifty-nine cases of melanoma (50 cases of skin primary melanoma, and 9 cases of metastatic melanoma) and 48 cases of melanocytic nevus (40 cases of common nevus and 8 cases of dysplastic nevus) were subject to PRAME immunohistochemistry staining.The difference of PRAME expression between melanoma and melanocytic nevus was analyzed.Results:Among the 50 patients with primary cutaneous melanoma, there were 23 males and 27 females ranging in age from 33 to 87 years (average age 62.4 years, median age 64.5 years). Among the 9 metastatic melanoma there were 7 males and 2 females ranging in age from 40 to 82 years (average age 64 years, median age 65 years). Twenty-six cases (26/50, 52.0%) of cutaneous primary melanoma and 4 cases (4/9) of metastatic melanoma showed diffuse positive PRAME staining. 40 cases (40/40, 100%) of common nevus and 8 (8/8) cases of dysplastic nevus were PRAME negative. Compared with melanocytic nevus group, the melanoma group included more cases with diffuse positive PRAME staining ( P<0.05). The sensitivity and specificity of using PRAME to differentiate primary cutaneous melanoma from melanocytic nevus in the cohort is 52.0% and 100%. Conclusions:There is a significant difference in the expression of PRAME between melanoma and melanocytic nevus.Thus, PRAME can be used as an auxiliary diagnostic tool for differentiating benign from malignant cutaneous lesions.
