The expression of miR-133a and miR-424 in serum and their relationship with T lymphocyte subsets in patients with connective tissue disease combined with interstitial lung disease
10.13431/j.cnki.immunol.j.20240048
- VernacularTitle:结缔组织病合并肺间质病变患者血清中miR-133a和miR-424表达及与T淋巴细胞亚群关系的研究
- Author:
Yue ZHAO
1
;
Jinliang YANG
;
Huan LUO
;
Wenxiu XI
;
Junlu WANG
;
Xuejun ZHENG
Author Information
1. 075000 张家口,河北北方学院附属第一医院风湿免疫科
- Keywords:
Connective tissue disease with interstitial lung disease;
miR-133a;
miR-424;
T lymphocyte subpopulation
- From:
Immunological Journal
2024;40(4):383-387
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of miR-133a and miR-424 in the serum of patients with connective tissue disease(CTD)and interstitial lung disease(ILD)and their relationship with T lymphocyte subpopulations,total of 96 CTD-ILD patients treated in our hospital from December 2019 to December 2022 were selected as CTD-ILD group,while 96 CTD patients without ILD were as the control group.The real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect serum levels of miR-133a and miR-424;flow cytometry was applied to detect the levels of T lymphocyte subpopulations.Pearson method was applied to analyze the relationship of miR-133a and miR-424 with T lymphocyte subpopulations.Compared with the control group,the level of serum miR-133a in the CTD-ILD group was obviously reduced,while the expression level of miR-424 was obviously increased(P＜0.05).Under different degrees of pulmonary ventilation disorders,the expression level of miR-133a in the serum of mild,moderate,and severe patients decreased obviously,while the expression level of miR-424 increased obviously(P＜0.05).Under different grades of pulmonary diffusion dysfunction,the expression level of miR-133a in the serum of mild,moderate,and severe patients reduced obviously,while the expression level of miR-424 increased obviously(P＜0.05).Furthermore,the levels of CD4+and CD4+/CD8+in the CTD-ILD group were obviously increased,as compared to the control group,while the levels of CD8+and CD3+were obviously reduced(P＜0.05).In addition,miR-133a was negatively correlated with CD4+,and positively correlated with CD8+and CD3+;miR-424 was positively correlated with CD4+,and negatively correlated with CD8+and CD3+(P＜0.05).In conclusion,the expression level of miR-133a in serum of CTD-ILD patients is decreased,while the expression level of miR-424 is increased,and both of them are related to the T lymphocyte subpopulations.
