Xuebijing injection inhibits TLR4 signaling pathway through up-regulating ADAM17 activity in EA.hy926 cells
10.13431/j.cnki.immunol.j.20240045
- VernacularTitle:血必净通过上调ADAM17酶活性抑制EA.hy926细胞TLR4信号通路
- Author:
Yingjie ZHANG
1
;
Jing WANG
;
Hongfei QI
;
Yalong KANG
;
Jing DONG
;
Yuanwang YU
;
Haifang WANG
Author Information
1. 712000,陕西中医药大学陕西省及咸阳市中西医结合心血管病防治重点实验室
- Keywords:
Xuebijing injection;
Vascular endothelial cell;
Inflammation;
TLR4;
ADAM17
- From:
Immunological Journal
2024;40(4):359-364
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to investigate the inhibitory effects of Xuebijing injection(XBJ)on lipopolysaccharide(LPS)-induced inflammatory signals on EA.hy926 vascular endothelial cells and the underlying mechanism,and to provide a theoretical basis for the treatment of sepsis with XBJ.WST-1 assay was used to detect the effects of XBJ on the cell viability;Western blot analysis was performed to detect the protein expression levels of IκBα,p-p65,p-ERK,p-JNK,p-p38,p-AMAD17 in cell lysates and the content of sTLR4 fragment in the concentrated culture supernatants.ADAM17 sheddase activity in cells was detected by using a commercial available kit.Data showed that all of the TLR4-mediated inflammatory signals were significantly inhibited by the treatment of XBJ(P＜0.01).ADAM17 phosphorylation and shedding activity were induced by XBJ treatment,simultaneously the sTLR4 contents in the culture media were increased.XBJ-induced shedding of TLR4 was suppressed by the preteatment of 10 μmol/L TAPI-1(an ADAM17 inhibitor).Taken together,XBJ can induce the shedding of TLR4 from cell membrane by up-regulating ADAM17 shedding activity,thereby inhibiting the activation of TLR4-mediated intracellular inflammatory signals in EA.hy926 cells.
